Download PDF, EPUB, MOBI Human Pluripotent Stem Cells
Download PDF, EPUB, MOBI Human Pluripotent Stem Cells

Human Pluripotent Stem Cells. Philip H. Schwartz

Human Pluripotent Stem Cells
---------------------------------------------------------------
Author: Philip H. Schwartz
Number of Pages: 488 pages
Published Date: 31 Aug 2011
Publisher: Humana Press Inc.
Publication Country: Totowa, NJ, United States
Language: English
Format: PDF
ISBN: 9781617792007
File size: 10 Mb
Download Link: human pluripotent stem cells
---------------------------------------------------------------


Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments. Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.

Read online Human Pluripotent Stem Cells Buy Human Pluripotent Stem Cells Download Human Pluripotent Stem Cells for pc, mac, kindle, readers Download to iPad/iPhone/iOS, B&N nook Human Pluripotent Stem Cells ebook, pdf, djvu, epub, mobi, fb2, zip, rar, torrent